In Vivo Acute Toxicity Evaluation of Desmidorchis Indica Stem Extract in Albino Rats

Abstract

Throughout human history, traditional herbal methods for treating diseases have been used without considering dose effects. The purpose of this study is to assess the acute toxicity of hydro-ethanolic extract of Desmidorchis indica (Family: Apocynaceae) in albino rats. Acute toxicity was achieved when a dose limit of 2000 mg/kg body weight was used. Observations were made, recorded 24 hours a day, and once a day for another 14 days. Rats were weighed and various observations were made once a day during this time, including signs of death, behavior, injury, and illness. At the end of each study, hematological analysis and biochemical parameters were evaluated. To detect histopathological examinations of the animal's vital organs (liver and kidneys) were performed compared to controls. No significant differences (p > 0.05) were observed in relative organ, weight, hematological, biochemical parameters, macroscopic abnormalities compared to controls. No mortality rate was recorded. Therefore, analysis of the results may lead to the conclusion that medium-term oral administration of Desmidorchis indica stem extract does not cause toxicity.