Curcumin Nanoparticles Incorporated Kumkumati Topical Agent for Gingival Depigmentation- An in Vitro Assessment of Antioxidant Activity

Abstract

Background: To date, no topical herbal formulations are available for the depigmentation of gingiva. The current study aims to formulate the herbal topical cream with curcumin nanoparticles incorporated into kumkumati oil followed by its assessment of antioxidant activity.
Materials and Method: 0.5mg of curcumin nanoparticles were mixed with one ml of dimethyl sulphoxide in a vortex mixture. To this five ml of kumkumati oil and 5mg of carbapol were added and mixed until the acquisition of creamy consistency. This was tested for antioxidant activity using a DPPH assay.
Result: The DPPH inhibition % of 25 μl, 50 μl, and 100 μl are 35.64 ± 0.32, 41.45 ± 0.44, 84.29 ± 0.56 respectively. This is comparable to the standard ascorbic acid which shows antioxidant activity of 98.79 ± 0.45.
Conclusion: The formulated material is shown to possess an increased antioxidant activity and thus can be used as a supplemental topical cream in post-surgical/laser gingival depigmentation periods to prevent repigmentation.