Assessment of Gene Expression of Some Human Cluster of Differentiation in Chronic HCV Patients

Abstract

Background: Hepatitis C virus (HCV) is one of the most potential pathogens all over the world. It is one of the main causes of chronic liver diseases. The host immune response against HCV sets off a chain reaction that results in liver damage after HCV infection in addition to necrosis and cellular inflammation, fibrosis, and hepatocellular carcinoma. Objective: This study aimed at evaluation of gene expression of human cluster of differentiation (CD25, CD39 and CD127) in patients with chronic HCV infection. Materials and Methods:  The study involved 70 HCV patients who were diagnosed in Hepatology and Gastroenterology Center at Azadi Teaching Hospital and 50 control subjects during October 2023 until April 2024. HCV patients were confirmed with real time PCR. In addition RNA extraction was conducted on patients and control whole blood to assess the gene expression of CD25, CD39 and CD127 in both groups. Result: Regarding gene expression of CD25, CD39 and CD127, our data revealed significant overexpression of those marker in HCV patients in comparison to control group with P value (0.0001, <0.0001 and <0.0001) respectively. Conclusion: The gene expression of CD25, CD39 and CD127 were significantly up-regulated in HCV patients compared to healthy control indicating the role played by these markers.