Prevention of Inflammation Through Increasing Interleukin-10 (IL-10) in Animal Models of Hyperglycemia

Abstract

Persistent high blood sugar levels lead to the malfunction and the decrease in the number of pancreas β cells that produce insulin leads to the initiation of inflammation inside the pancreas. It has been shown that the development of diabetes mellitus is associated with inflammation as well as the stimulation of the intrinsic immunity systems. Increased IL-10 expression plays a role in balancing proinflammatory cytokines that limit tissue damage. This research seeks to evaluate the impact of mangosteen peel extract on preventing inflammation by boosting IL-10 levels in hyperglycemic animal models. The study was conducted as a laboratory experiment, using a distinct pretest-posttest control group design that is randomly. Negative control (KN), positive control with STZ (K1), STZ and CMC-Na (K2), mangosteen peel extract at 200 mg/kg body weight (K3), 400 mg/kg body weight (K4), and 600 mg/kg body weight (K5) were the six groups that were included in the sample. Additionally, the sample was composed of male white rats from the Wistar strain. The treatment was given regularly for 14 days. The research revealed that rats administered with mangosteen peel extract had a significantly greater quantity of IL-10 positive cells, averaging 7.66, in contrast to the uncontrolled group which had an average of 2.44. The number of IL-10-expressing cells differed significantly (p < 0.05) across all treatment groups. The group administered with a dosage of 600 mg/kg BW/day of mangosteen peel extract had the greatest count of IL-10-positive cells, demonstrating superior effectiveness compared to the other dosages. This study concludes that different doses of mangosteen peel extract influence the increase in IL-10-positive cells and help prevent inflammation in hyperglycemia, with 600 mg/kg BW/day being the most effective. The findings of this study emphasize the properties of mangosteen peel and indicate that it may have the potential to serve as an alternative therapy for illnesses that are associated with inflammation in humans.