The Effect Of Iron Citrate Synthesit On Cognitive Functions In Mice

Abstract

Background: This study evaluated the effect of iron citrate synthesit on cognitive functions in SHK strain mice, focusing on spatial memory and learning efficiency.

Methods and Results: Ten male SHK strain mice (8-10 weeks old, 20-25 g) were randomly divided into two groups of five: a control group and an experimental group. The experimental group received 50 μg/day of iron citrate in drinking water for 6 days. Cognitive functions were assessed using an eight-arm radial maze over a 6-day period, including habituation, training, and testing phases, with a break on day 5 to evaluate long-term memory. Parameters measured included correct and incorrect entries, maze completion time, and memory scores. By day 6, the experimental group showed statistically significant improvement in spatial memory (p<0.05), with a mean memory score of -0.19 compared to -0.38 in controls. Maze completion time decreased by 30% (from 322 to 295 seconds), compared to an 18% decrease in the control group (316 to 305 seconds). After the break, the experimental group demonstrated better memory retention (mean correct entries: 3.2 vs. 2.8; p>0.05).

Conclusions: Iron citrate Synthesit at 50 μg/day enhances cognitive function in SHK mice, improving spatial memory, learning speed, and retention in maze-based tasks.