FTIR Based Phytochemical Profiling And Antibacterial Evaluation Of Green Synthesized Agnps, Cunps And Bimetallic Ag-Cunps Using Aqueous Stem Extract Of Achyranthes Aspera

Abstract

Researchers now-a-days continue to face significant challenges in developing effective antibacterial drugs, as microorganisms increasingly develop resistance to existing antibiotics. The synthesis of nanoparticles from plant extracts represents a significant progress in the field Nanotechnology. Our present study focuses on green synthesis of Metallic nanoparticles (MNPs)- AgNPs, CuNPs and bimetallic Ag-Cu NPs using aqueous stem extract of Achyranthes aspera, chosen for its non-toxic, cost-effectiveness and eco-friendly nature. As a preliminary step, phytochemical analysis of Achyranthes aspera stem extract was conducted to identify the presence of phyto-constituents such as Phenolic compounds, flavonoids, alkaloids, tannins, which plays a critical role in the reducing, capping, and stabilizing the nanoparticles. Fourier Transform Infra-Red (FTIR) spectroscopy analysis was employed to profile these phytochemicals. The antimicrobial activity was evaluated at various concentrations against Gram positive bacteria (Staphylococcus aureus and Streptococcus pneumonia) and Gram-negative bacteria (Pseudomonas aeruginosa and E. coli) using Agar Well Diffusion Assay. Anti-bacterial efficacy was assessed by measuring the diameter of the inhibition zones (ZOI). The microbial activity of the bimetallic Ag-Cu NPs was found efficient against all the tested pathogens, therefore, has a promising utility of green synthesized nanoparticle as an alternative to standard antibiotics, mainly in preventing multi-drug-resistant bacterial strains.