Bacopa Monnieri Bacterial Endophytes: Regulating Plant Growth And Active Phytochemicals

Abstract

The alteration of rhizospheric and endophytic diversity of microorganisms are of increasing attention as shift in microbic community structure influences the reciprocal function of host-microbiome relations. The present study investigates the previously unexplored dominion of endophytic bacterial partners of medicinal plant Bacopa monnieri and discloses their crucial function in endorsing plant growth and biosynthesizing active phyto-ingredients. Two bacterial isolates (Achromobacter denitrificans and Shinella oryzae) were selected and identified using phenotypic and and molecular characterization from an extensive collection of bacterial isolates. The synergetic potential of bacterial endophytes was validated by the noted growths in B. monnieri's shoot and root length after inoculation. On the basis of LC-MS analysis, several active phyto-ingredients, such as bacopaside I, II, brahmic acid, epigenin, eblin, and stigmasterol, were observed in appreciably higher content in endophytes inoculated treatments. These phytochemicals were detected in an experiment conducted in sterile soil, emphasizing the intricate interactions between host plant and bacterial endophytic community. This report first time mention the role of endophytic bacteria Achromobacter denitrificans and Shinella oryzae in enhancing the B. monnieri plant growth and active ingredients. This pioneering unearthing brings up new prospects for sustainable agriculture and pharmacological improvement as well as revealing the previously unidentified potential of B. monnieri's endophytic associates.