Higher insulin resistance is associated with decreased expression of the vitamin D receptor gene in obese type II diabetic patients with vitamin D deficiency

Abstract

The Background: The etiology of numerous medical conditions, such as metabolic irregularities, is interconnected with the insufficiency of vitamin D. Furthermore, a theoretical association between the resistance to insulin and the insufficiency of vitamin D has been postulated. This concept is substantiated by empirical evidence obtained through various investigations. The objective of this investigation is to analyze and investigate the significance and influence of vitamin D and the expression of the vitamin D receptor (VDR) gene in the progression of insulin resistance in individuals with T2DM.

Methods: The study was carried out 100 type II diabetic patients and 100 healthy controls. Both groups had anthropometry examinations to determine their weights, heights, hip and waist circumferences. Fasting glucose, insulin, insulin resistance (HOMA-IR), and vitamin D levels were all measured. Quantitative real-time PCR was used to analyze the expression of VDR genes in both groups. The folding change was computed using the conventional 2^-(∆∆ct) technique.

Results: T2DM patients had higher levels of all anthropological measurements, and biochemical parameters compared to controls. Patients had lower levels VDR folding change and vitamin D levels. The diagnostic accuracy of anthropological assessments and biochemical parameters in prediction disease showed significant results. VDR gene expression has a highly significant negative linear association with insulin resistance in obese with vitamin D deficiency patients. Also, negative linear correlation noted between insulin resistance and serum vitamin D levels without significant variation.

Conclusion: VDR gene expression and insulin resistance were connected in obese patients T2DM with vitamin D insufficiency..