Isolate and Molecular Identification of Bundle Forming Pilus Gene for Escherichia coli Caused Caw Mastitis

Abstract

Mastitis is one of the disease infected Caws and effect of their milk product lead economic loss. The aim of this study isolated and molecular identification Bundle Forming Pilus Gene for Escherichia coli caused Caw mastitis. Samples were collected from Caws infected with Mastitis at different farm in Iraq, then applied for cultivation on the suitable media for isolated of E. coli. The E.coli  isolates was confirmed by VITEK II automated microbial identification system method and identification by PCR method for expression of bundle forming pilus gene. The result was shown that (9 from 100) from mastitis cases give E. coli positive. All E.coli  isolates were confirmed by VITEK method. All E.coli  isolates were expressed of bundle forming pilus gene. Conclusion: E.coli  caused severe mastitis in Caw and the severity of infection come from express of bundle forming pilus gene that increases the virulence of E.coli  be more resistance to antibiotics and unsuitable conditions that may be lead to many serious diseases if dietary contaminated with mastitis E.coli .