Immunogenic Detection of IL-6 in Male Patients Suffering from Infertility Disorders

Abstract

Background; Infertility in men is one of the health and psychological problems around the world that is widespread and related to many physiological and medical causes. Many hormonal differences or viral or bacterial infections in general can lead to infertility in men. Aims of the study; The effect of interleukin 6 and its role in the development of male infertility. Methodology; This study was conducted on patients at Al-Sadr Medical City / Infertility Center / Al-Najaf Al-Ashraf and Dr. Ali Al-Ibrahimi's private center for embryology and infertility. Since October 2023, the control group (fertile men) included 40 healthy 25–45-year-old men. There were 60 infertile men aged 22-46 in the study. An enzyme-linked immunosorbent assay measured interleukin-6. DNA extraction was followed by PCR analysis of the interleukin 6 gene polymorphism. Result; The results showed that there was no statistical significance in age between the two groups. The results also showed an increase in interleukin 6 levels in general and by age groups in the patient group compared to the control group. In addition there is a statistical significance in the polymorphism gene tests between the two groups. Conclusions; The case group had higher interleukin 6 levels, and the gene polymorphism was significant. This suggests that inflammatory factors like viral or bacterial infections or autoimmunity caused the difference in interleukin 6 levels and the mutation in IL-6 gene could play a role in this increase.