Study the Different Antioxidants Effects on Serum Quality in Dogs

Abstract

Dogs frequently have subfertility linked to poor semen quality (high percentage of morphologically defective spermatozoa, low rate of gradual mobility and total number of sperm .To examine the anti-oxidative effect of  various concentratuons of NAC on cryopreservation in dogs sperm.The animals are divided into three groups: C0, C1  and C2. C0 receives the 0mM NAC, C1 receives 0.35mM and C2 receives 0.85mM. The physical characteristics studied of the spêrm include ejaculated volume, semen color, motility of sperms, percentage of dead sperms and abnormal sperms. After physical evaluation, the samples are diluted with Tri’s diluter. The tris diluter is egg-yolk glycerol. The samples are then cryopreserved in liquid nitrogen (-196 C) from October to December.  A total of 50 samples are calculated and prepared for freezing in the following way:The motility of sperm is high for 0.35mM dose of NAC,  and not very different in the control group and 0.85mM NAC group. The motility is significantly different for 0.35NAC as compared to the other two groups. The p-value (< 0.05) shows the significant difference between the motility of 0.35 NAC and other groups. The sperm viability is highest in the 0.35mM NAC group (30.34). The viability of sperm is highest at 0.85mM. The membrane integrity is highest in 0.35mM NAC, while lowest in the control group( p<0.05).In conclusion, dogs sperm cells can be shielded from oxidative stress by using moderate dosages of NAC supplied in Tris extender without negatively affecting dog semen's ability to freeze.