Investigation of Introns in Proteus Mirabilis Bacteria and Extent of their Resistance to Antibiotics

Abstract

Proteus mirabilis is an opportunistic pathogen meaning that causes infection in people with reduced natural immunity. These bacteria possess many virulence factors or pathogens which is essential for colonizing new areas, including tissues and hosts. The study aimed to investigate phenotypically or genetically the introns (MIs) in P. mirabilis by estimate the extent of its resistance to antibiotics. The results showed that the appearance of genetic packages for all migrated isolates at one level. This is an indication of the binding of the primer to its complementary sequence in the DNA strand. The molecular weights of the resulting genetic packages were estimated based on known standard packages, the apparent molecular weights in the path (M) with a graduated size (100- 200 bp). The results also showed a clear similarity of the molecular weight of the resulting bands (1500 bp) with the molecular weight of the diagnostic gene rRNA 16s. In addition, 20 isolates were examined using PCR technology for the three classes of introns gene. All isolates were carried the chromosome intron gene. The appearance of the intron 1 gene on all isolates was (100%).