Phytochemical, Antioxidant And Physicochemical Evaluation Of Holoptelea Integrifolia (Roxb.) Seed Oil

Abstract

Holoptelea integrifolia (Roxb.) from the ulmaceae family, a useful medicinal plant possess number of traditional medicinal uses related to its potential phytochemical constituents and antioxidant activity. Folk use various parts of this plant like leaves, barks and seeds to treat different disease conditions. These ailments include inflammation, rheumatism, skin and eye infections and urinary tract disorders like poly urea. The current work was planned to explore the bioactive phytoconstituents present in the seed oil of this plant and its antioxidant activity using a DPPH-Radical Scavenging Assay (DPPH-RSA) . The DPPH-RSA assessed that the seed oil had significant antioxidant activity. Moreover, the detection of phenolic and flavonoid content further highlights the antioxidant potential of the seeds oil. Phytochemical screening was conducted using three spectroscopic techniques i.e., Fourier Transform Infrared Spectroscopy (FTIR), Gas Chromatography Flame Ionization Detection (GC-FID), and Gas Chromatography Mass Spectrometry (GCMS). The FTIR spectra revealed characteristic functional groups, including aliphatic chains, esters, aldehydes, and phenols. In GC-FID analysis, thirteen fatty acids were identified. While the GCMS identified 15 compounds, including alkanes, aldehydes, alcohols, carboxylic acids, and phenolic compounds. Notable bioactive constituents include palmitic acid, octadec-9-enoic acid, and (E)-2-Octenal, known for their medicinal properties. Key physicochemical properties, including iodine value and saponification value, were also analyzed.

These results underscore that the seed oil of this plant is a potential candidate for further investigations and can be a rich source of bioactive constituents for the development of ethno medicine.