Increased Runx-2 Expression In The Y-TZP Biomaterial Osteointegration Process With hADMSC Seeding

Abstract

Introduction:. Peri-implantitis, overload, and poor prosthetic construction result in dental implant failure. The success factor of the implant is osteointegration, the formation of tissue between the implant surface and the bone without fibrous tissue. Human Mesenchymal bone marrow (hBMSC) is difficult and risky. Biomarkers associated with increased osteogenesis for osseointegration of biomaterials. This research was conducted to analyze biomarkers that can occur osteogenesis, to increase osteointegration of biomaterials that have high biocompatibility using mesenchymal stem cells. Methods: The research carried out is a true experimental study on Wistar rat test animals totaling 6 treatment groups consisting of 3 groups at the 1st week and 8th week. Each tissue sample was made into a slice preparation with a thickness of 4um, then detected by immunohistochemical examination for Runx2 expression and data in analysis. Results: An increase in Runx-2 expression occurred significantly at week 8 in the Y-TZP biomaterial treatment group seeded with hADMSC (P4). Disscusion: Through increased expression of Runx-2 is a biomarker as a marker on hADMSC which can increase osteogenesis and osteointegration of Y-TZP biomaterial. The use of Y-TZP biomaterial in tissue engineering can be used in other fields of medicine, due to its good biocompatibility as an implant biomaterial so that implant failure can be prevented. Conclusion: Dental implant failure prevented by hADMSC seeding in Y-TZP via increased Runx-2 extrusion. Runx-2 in osteogenesis and fibroblast migration are influenced by various genes in the bone healing process. The benefits of this research can reduce the stiffness of dental implants.